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reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 373-381 doi: 10.15302/J-FASE-2018210

摘要：

Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.

关键词： Aspergillus oryzae     egg allergy     egg white proteins     IgE-binding ability     ovomucoid    

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Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from

Yuan ZHANG,Yan ZHANG,Tao YU

《环境科学与工程前沿（英文）》 2014年 第8卷 第5期   页码 666-674 doi: 10.1007/s11783-013-0608-y

摘要： Dissolved organic matter (DOM) plays an important role in heavy metal speciation and distribution in the aquatic environment especially for eutrophic lakes which have higher DOM concentration. Taihu Lake is the third largest freshwater and a high eutrophic lake in the downstream of the Yangtze River, China. In the lake, frequent breakout of algae blooms greatly increased the concentration of different organic matters in the lake sediment. In this study, sediment samples were collected from various part of Taihu Lake to explore the spatial difference in the binding potential of DOM with Cu. The titration experiment was adopted to quantitatively characterize the interaction between Cu(II) and DOM extracted from Taihu Lake sediments using ion selective electrode (ISE) and fluorescence quenching technology. The ISE results showed that the exogenous DOM had higher binding ability than endogenous DOM, and DOM derived from aquatic macrophytes had a higher binding ability than that derived from algae. The fluorescence quenching results indicated that humic substances played a key role in the complexation between DOM and Cu(II) in the lake. However, because of the frequent breakout of algae blooms, protein-like matters are also main component like humic matters in Taihu Lake. Therefore, the metals bound by protein-like substances should be caused concern as protein-like substances in DOM were unstable and they will release bound metal when decomposed.

关键词： binding ability     dissolved organic matters     fluorescence quenching     complex capacity     Taihu Lake    

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

《医学前沿（英文）》 2022年 第16卷 第3期   页码 378-388 doi: 10.1007/s11684-021-0840-y

摘要： Macrolide and corticosteroid resistance has been reported in patients with Mycoplasma pneumoniae (MP) pneumonia (MPP). MP clearance is difficult to achieve through antibiotic treatment in sensitive patients with severe MPP (SMPP). SMPP in children might progress to airway remodeling and even bronchiolitis/bronchitis obliterans. Therefore, identifying serum biomarkers that indicate MPP progression and exploring new targeted drugs for SMPP treatment require urgency. In this study, serum samples were collected from patients with general MPP (GMPP) and SMPP to conduct proteomics profiling. The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of SMPP. Biological enrichment analysis indicated uncontrolled inflammation in SMPP. ELISA results proved that the FCGBP level in patients with SMPP was substantially higher than that in patients with GMPP. Furthermore, the FCGBP levels showed a decreasing trend in patients with GMPP but the opposite trend in patients with SMPP during disease progression. Connectivity map analyses identified 25 possible targeted drugs for SMPP treatment. Among them, a mechanistic target of rapamycin kinase (mTOR) inhibitor, which is a macrolide compound and a cell proliferation inhibitor, was the most promising candidate for targeting SMPP. To our knowledge, this study was the first proteomics-based characterization of patients with SMPP and GMPP.

关键词： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein     mechanistic target of rapamycin kinase inhibitor    

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Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

《结构与土木工程前沿（英文）》 2016年 第10卷 第1期   页码 112-120 doi: 10.1007/s11709-015-0322-x

摘要： Corrosion of embedded rebars is a classical deterioration mechanism of reinforced concrete structures exposed to chloride environments. Such environments can be attributed to the presence of seawater, deicing or sea-salts, which have high concentrations of chloride ion. Chloride ingress into concrete, essential for inducing rebar corrosion, is a complex interaction between many physical and chemical processes. The current study proposes two chloride ingress parameter models for fly ash concrete, namely: 1) surface chloride content under tidal exposure condition; and 2) chloride binding. First, inconsistencies in surface chloride content and chloride binding models reported in literature, due to them not being in line with past research studies, are pointed out. Secondly, to avoid such inconsistencies, surface chloride content and chloride binding models for fly ash concrete are proposed based upon the experimental work done by other researchers. It is observed that, proposed models are simple, consistent and in line with past research studies reported in literature.

关键词： binding isotherms     chloride ingress     concrete     fly ash     surface chloride content    

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Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block

null

《医学前沿（英文）》 2016年 第10卷 第4期   页码 420-429 doi: 10.1007/s11684-016-0478-3

摘要：

Inappropriate cell proliferation during oncogenesis is often accompanied by inactivation of components involved in the cell cycle machinery. Here, we report that cyclin-dependent kinase inhibitor 2C (CDKN2C) as a member of the cyclin-dependent kinase inhibitors is a target of the PML/RARα oncofusion protein in leukemogenesis of acute promyelocytic leukemia (APL). We found that CDKN2C was markedly downregulated in APL blasts compared with normal promyelocytes. Chromatin immunoprecipitation combined with quantitative polymerase chain reaction demonstrated that PML/RARα directly bound to the CDKN2C promoter in the APL patient-derived cell line NB4. Luciferase assays indicated that PML/RARα inhibited the CDKN2C promoter activity in a dose-dependent manner. Furthermore, all-trans retinoic acid treatment induced CDKN2C expression by releasing the PML/RARα binding on chromatin in NB4 cells. Functional studies showed that ectopic expression of CDKN2C induced a cell cycle arrest at the G0/G1 phase and a partial differentiation in NB4 cells. Finally, the transcriptional regulation of CDKN2C was validated in primary APL patient samples. Collectively, this study highlights the importance of CDKN2C inactivation in the abnormal cell cycle progression and differentiation block of APL cells and may provide new insights into the study of pathogenesis and targeted therapy of APL.

关键词： CDKN2C     acute promyelocytic leukemia     cell cycle arrest     differentiation    

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Filtration ability of hollow fiber membrane for production of magnesium ammonium phosphate crystals by

H. Watamura, H. Marukawa, I. Hirasawa

《化学科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 55-59 doi: 10.1007/s11705-013-1312-y

摘要： Relationship between magnesium ammonium phosphate (MAP) crystal properties and the filtration ability of hollow fiber membrane (HFM) were investigated. Phosphorus recovery process by crystallization has a problem that it produces a large amount of fine crystals. So improvement of the crystallization process by combining with filtration was discussed. MAP crystals were obtained by batch reaction crystallization and the filtration characteristics were investigated. The filtration was evaluated by the specific filtration resistance ( ) on HFM. Filtered slurry was prepared with each suspension density and crystal size distribution. The solution was filtered at constant pressure of 0.02 MPa and the filtration time on each filtrated volume was recorded. As a result, decreases exponentially with suspension density increasing from 0.25 g/L to 0.5 g/L and decreases moderately with suspension density increasing from 0.5 g/L to 1.5 g/L. of large crystals decreases exponentially at less suspension density than of small crystals does. Also, increases as the ratio of the fractured crystals increases.

关键词： membrane separation     crystallization     MAP    

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一类弱非线性波浪数值模型及其适用性分析

刘忠波,唐军

《中国工程科学》 2010年 第12卷 第9期   页码 96-100

摘要：

研究了一类含弱非线性的改进型Boussinesq水波方程，在非交错网格下，利用有限差分法建立了混合四阶Adams-Bashforth-Moulton的预报校正格式的波浪数值模型。在数值模型中，关于空间一阶导数差分格式采用四阶精度、二阶导数差分格式采用二阶精度。针对波浪的一维、二维传播变形问题进行了数值计算，并通过与相关实验结果对比分析考察了该数值模型的适用性。

关键词： 数值模型     适用性     波浪    

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优化输水方式提高三峡船闸通过能力措施研究

曹光荣,胡亚安,高雄

《中国工程科学》 2007年 第9卷 第12期   页码 75-80

摘要： 探讨了三峡船闸四级运行方式下二闸首作为首级运行时一闸室水体波动特性；阐述了波动形成的机理，分析了二闸首阀门运行方式对一闸室波动的影响；提出了满足一闸室待闸安全要求的阀门开启方式；通过实船试验检验了一闸室待闸船舶停泊条件，总结了一闸室待闸调度方式。运行实践表明，三峡船闸完建期实行四级运行方式下，将下行船舶由靠船墩移至一闸室满闸室待闸，可提高船闸日均通过闸次近3个，通航效益明显。

关键词： 三峡船闸；输水方式；水流条件；通过能力    

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Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction with

null

《医学前沿（英文）》 2014年 第8卷 第2期   页码 217-226 doi: 10.1007/s11684-014-0326-2

摘要：

This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>104 copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.

关键词： hepatocellular carcinoma (HCC)     interaction     miRNA-122-binding site     IL-1A     rs3783553     hepatitis B virus (HBV) mutations    

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Diffusive gradients in thin films using molecularly imprinted polymer binding gels for in situ measurements

Ying Cui, Feng Tan, Yan Wang, Suyu Ren, Jingwen Chen

《环境科学与工程前沿（英文）》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1290-5

摘要： Abstract • Selective molecularly imprinted polymer (MIP) binding gel was prepared. • MIP-DGT showed excellent uptake performance for antibiotics. • In situ measurement of antibiotics in wastewaters via MIP-DGT was developed. • The MIP-DGT method was robust, reliable, and highly sensitive. Urban wastewater is one of main sources for the introduction of antibiotics into the environment. Monitoring the concentrations of antibiotics in wastewater is necessary for estimating the amount of antibiotics discharged into the environment through urban wastewater treatment systems. In this study, we report a novel diffusive gradient in thin films (DGT) method based on molecularly imprinted polymers (MIPs) for in situ measurement of two typical antibiotics, fluoroquinolones (FQs) and sulfonamides (SAs) in urban wastewater. MIPs show specific adsorption toward their templates and their structural analogs, resulting in the selective uptake of the two target antibiotics during MIP-DGT deployment. The uptake performance of the MIP-DGTs was evaluated in the laboratory and was relatively independent of solution pH (4.0–9.0), ionic strength (1–750 mmol/L), and dissolved organic matter (DOM, 0–20 mg/L). MIP-DGT samplers were tested in the effluent of an urban wastewater treatment plant for field trials, where three SA (sulfamethoxazole, sulfapyridine, and trimethoprim) and one FQ (ofloxacin) antibiotics were detected, with concentrations ranging from 25.50 to 117.58 ng/L, which are consistent with the results measured by grab sampling. The total removal efficiency of the antibiotics was 80.1% by the treatment plant. This study demonstrates that MIP-DGT is an effective tool for in situ monitoring of trace antibiotics in complex urban wastewaters.

关键词： Diffusive gradients in thin films     Molecularly imprinted polymers     Selective uptake     Antibiotics     Passive sampling    

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Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF

Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 362-367 doi: 10.1007/s11783-015-0803-0

摘要： Interactions between metals and activated sludge can substantially affect the fate and transport of heavy metals in wastewater treatment plants. Therefore, it is important to develop a simple, fast and efficient method to elucidate the interaction. In this study, a modified titration method with a dynamic mode was developed to investigate the binding of Cu(II), a typical heavy metal, onto aerobic granules. The titration results indicated that pH and ionic strength both had a positive effect on the biosorption capacity of the granular sludge. The -XRF results demonstrated that the distribution of metals on the granular surface was heterogeneous, and Cu showed strong correlations and had the same “hot spots” positions with other metal ions (e.g., Ca, Mg, Fe etc.). Ion exchange and complexing were the main mechanisms for the biosorption of Cu(II) by aerobic granules. These results would be beneficial for better understanding of Cu(II) migration and its fate in wastewater treatment plants.

关键词： aerobic granules     Cu(II)     modified titration     µ-XRF analysis    

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3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films with enhanced electrocatalytic ability

《化学科学与工程前沿（英文）》 2023年 第17卷 第6期   页码 735-748 doi: 10.1007/s11705-022-2262-z

摘要： In this study, a simple and effective method was proposed to improve the electrocatalytic ability of overoxidized poly(3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films modified on glassy carbon electrode for rutin and luteolin determination. The composite electrode was prepared by cyclic voltammetry copolymerization with LiClO4-water as the supporting electrolyte. The peak current of rutin and luteolin on the composite electrode gradually decreased or even disappeared with the increase in the positive potential limit. After incubation in NaOH–ethanol solution with a volume ratio of 1:1, the composite electrodes prepared at positive potential limit greater than 1.5 V exhibited enhanced differential pulse voltammetry peak currents, reduced charge transfer resistance, larger effective specific surface area and higher electron transfer rate constant. The composite electrode prepared in the potential range of 0–1.7 V showed optimal electrocatalytic performance. The X-ray photoelectron spectroscopy results indicated that the content of –SO2/–SO and –C=N– groups in the composite film increased significantly after incubation. Further, the Raman spectra and Fourier transform infrared spectra revealed that the thiophene ring structure changed from benzene-type to quinone-type, and the quinone-type pyrrole ring was formed. The electrocatalytic mechanism of the composite film was proposed based on the experimental results and further verified by Density Functional Theory calculation.

关键词： overoxidized poly(3     4-ethylenedioxythiophene)-overoxidized polypyrrole     rutin     luteolin     incubation     electrocatalytic mechanism    

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Fabrication of titania thin film with composite nanostructure and its ability to photodegrade rhodamine

ZHANG Gaojie, WU Jinming, LIU Shaoguang, YAN Mi

《化学科学与工程前沿（英文）》 2008年 第2卷 第1期   页码 44-48 doi: 10.1007/s11705-008-0001-8

摘要： A titania nanorod film was synthesized by direct oxidation of metallic Ti with hydrogen peroxide solution under a low temperature. Titania nanoparticles were then filled into the gaps among the nanorods through an infiltration sol-gel procedure to form a composite titania film with an ordered nanostructure. X-ray diffraction spectra indicate that the composite film was a mixture of anatase and rutile while the titania film obtained by only using a sol-gel procedure was pure anatase. Field emission scanning electron microscopy observations show that titania nanoparticles were embedded into the titania nanorod film. Photoluminescence spectra suggest the enhanced separation of electron and hole pairs for the obtained composite titania film over the corresponding titania nanorod film. The composite titania film exhibited improved ability to photodegrade rhodamine B in water compared with the titania nanorod film. The apparent photodegradation rate constant, fitting a pseudo-first-order, was 3 times of that obtained by the sol-gel derived titania film at the same weight. The improved photocatalytic activity for the composite titania film could be attributed to the enhanced separation of electron and hole pairs due to the embedding of the titania nanoparticles within the titania nanorods.

关键词： constant     weight     apparent photodegradation     hydrogen     nanorod    

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Atomistic characterization of binding modes and affinity of peptide inhibitors to amyloid-

Fufeng LIU,Wenjie DU,Yan SUN,Jie ZHENG,Xiaoyan DONG

《化学科学与工程前沿（英文）》 2014年 第8卷 第4期   页码 433-444 doi: 10.1007/s11705-014-1454-6

摘要： The aggregation of amyloid -protein (A ) is tightly linked to the pathogenesis of Alzheimer’s disease. Previous studies have found that three peptide inhibitors (i.e., KLVFF, VVIA, and LPFFD) can inhibit A aggregation and alleviate A -induced neurotoxicity. However, atomic details of binding modes and binding affinities between these peptide inhibitors and A have not been revealed. Here, using molecular dynamics simulations and molecular mechanics Poisson Boltzmann surface area (MM/PBSA) analysis, we examined the effect of three peptide inhibitors (KLVFF, VVIA, and LPFFD) on their sequence-specific interactions with A and the molecular basis of their inhibition. All inhibitors exhibit varied binding affinity to A , in which KLVFF has the highest binding affinity, whereas LPFFD has the least. MM/PBSA analysis further revealed that different peptide inhibitors have different modes of interaction with A , consequently hotspot binding residues, and underlying driving forces. Specific residue-based interactions between inhibitors and A were determined and compared for illustrating different binding and inhibition mechanisms. This work provides structure-based binding information for further modification and optimization of these three peptide inhibitors to enhance their binding and inhibitory abilities against A aggregation.

关键词： Alzheimer’s disease     amyloid β-protein     peptide inhibitors     protein-protein interaction     molecular dynamics simulation    

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基于微笑曲线理论的中国建筑业核心竞争力研究

叶娟,刘宏蛟

《中国工程科学》 2008年 第10卷 第12期   页码 86-91

摘要：

建国以来，中国建筑业产值一直稳步提高，但低效率、低收益问题仍然没有得到解决。基于拓展服务领域、增加附加价值以提高我国建筑业核心竞争力的思路，从“广义的建筑业”和“广义的建设项目生命周期”出发，构建了建筑业的工程价值体系，并定义了相对附加经济价值这一概念。根据对目前我国建筑业现状的分析结果，研究了建设项目生命周期各个不同阶段附加价值的增值特点，做出了我国不同时期建筑业的微笑曲线，预测了我国建筑业未来的发展方向，最后探讨了提升我国建筑业核心竞争力的上、中、下游和整体发展战略。

关键词： 建筑业     核心竞争力     微笑曲线     相对附加经济价值     全生命周期    

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标题 作者 时间 类型 操作

reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

期刊论文

Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from

Yuan ZHANG,Yan ZHANG,Tao YU

期刊论文

Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

期刊论文

Chloride binding and time-dependent surface chloride content models for fly ash concrete

S. MUTHULINGAM,B. N. RAO

期刊论文

Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block

null

期刊论文

Filtration ability of hollow fiber membrane for production of magnesium ammonium phosphate crystals by

H. Watamura, H. Marukawa, I. Hirasawa

期刊论文

一类弱非线性波浪数值模型及其适用性分析

刘忠波,唐军

期刊论文

优化输水方式提高三峡船闸通过能力措施研究

曹光荣,胡亚安,高雄

期刊论文

Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction with

null

期刊论文

Diffusive gradients in thin films using molecularly imprinted polymer binding gels for in situ measurements

Ying Cui, Feng Tan, Yan Wang, Suyu Ren, Jingwen Chen

期刊论文

Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF

Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG

期刊论文

3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films with enhanced electrocatalytic ability

期刊论文

Fabrication of titania thin film with composite nanostructure and its ability to photodegrade rhodamine

ZHANG Gaojie, WU Jinming, LIU Shaoguang, YAN Mi

期刊论文

Atomistic characterization of binding modes and affinity of peptide inhibitors to amyloid-

Fufeng LIU,Wenjie DU,Yan SUN,Jie ZHENG,Xiaoyan DONG

期刊论文

基于微笑曲线理论的中国建筑业核心竞争力研究

叶娟,刘宏蛟

期刊论文